Reimagine Protein Biomarker Quantitation
Achieve ultrasensitive biomarker quantitation using SPLASH™ (Solid Phase Ligation Assay with Single wasH), a simple workflow accessible to any lab
Maximizing protein biomarker signals from blood with SPLASH™
Protein biomarkers like pTau-217 are often present in blood at extremely low levels – levels that, while clinically significant, remain undetectable by many existing assays.
Our proprietary SPLASH™ (Solid Phase Ligation Assay with Single wasH) technology dramatically enhances the sensitivity and specificity of biomarker detection, enabling more accurate and reliable biomarker measurement. Beyond pTau-217, our chemistry easily adapts to a wide range of protein biomarkers.
What is SPLASH?
A novel dual-conjugated antibody probe
While conventional PLA utilizes oligo-conjugated antibodies, our SPLASH technology incorporates an additional binding moiety onto one antibody in the probe pair. This enables selective capture of the formed immunocomplex.
Unique one-wash PLA workflow
Unbound probes and interfering substances are washed away, while the immunocomplex is captured and quantified.
Ultrasensitivity with a simple workflow
Our highly scalable workflow can be fully automated or performed manually, requiring no additional instruments.
How does it work?
Antibody probes and target protein bind to form an immunocomplex
Immunocomplexes are captured via a biotin-streptavidin interaction
Unbound molecules from the sample matrix are washed away
Two unique oligos on the antibody probes are ligated
The ligation is inactivated and DNA is released
The DNA is quantified with qPCR
See the data from our pTau-217 wash-based proximity ligation assay
Ultrasensitive
Reference curve shows linearity of pTau-217 detection down to fg/mL concentrations.
Highly specific
Spike and recovery of pTau-217 falls within a range of 80-120%.
Accurate
Measured pTau-217 clearly distinguishes between positive and negative AD patient samples.
Reliable protein quantification on any bench at any budget
Incubate
1 hour
Wash & Ligate
5-10 mins
qPCR
1 hour
Simplify AD biomarker detection with NeuXplore™, our fully automated sample-to-answer solution
Scalable sample-to-answer Dx system
