Alzheimer's Disease Biomarker Testing Made Simple

Easily test for pTau-217, Aβ42, Aβ40, NfL, and GFAP from a simple blood draw using our ultrasensitive SPLASH™ kits.

Blood-based biomarkers are redefining Alzheimer’s disease diagnostics

Recent advances in blood-based biomarkers are transforming Alzheimer’s diagnostics. The science has matured to a point where a simple blood test can provide clinically meaningful insight into amyloid and tau pathology, with performance validated against established platforms.

Our SPLASH™ platform, validated across dynamic range, signal-to-background, and dilution linearity, provides the ultrasensitive needed to quantify Alzheimer’s disease most relevant biomarkers in blood.

Easily quantify blood-based protein biomarkers on your existing qPCR instrument

No new capital equipment, no specialized training. SPLASH™ runs on the qPCR instrument sitting in your lab today, so you can start testing immediately.

  • Simple 3-step benchtop workflow (incubate, wash, qPCR)
  • Less than 30 minutes of hands-on time
  • Works on standard instruments, including QuantStudio 5/7/12, BioRad Opus, and Roche LightCycler

Getting started is easy. Order the kit(s) that best suit your needs, read the Quick Guide, and receive results in less than 30 minutes of hands-on time.

Feature Taudia SPLASH Simoa MSD ELISA
Uses existing qPCR
Ultrasensitive ~ ~
Multiplex capability (5+) Limited (10+) Limited
Specialized instrument No Yes Yes No
Workflow complexity Low Moderate Moderate Moderate
Cost per sample Low High Moderate Low

5-plex protein profiling from Alzheimer’s Disease using only 12.5 ul of plasma

SPLASH™’s 5-plex panel measures pTau-217, Aβ42, Aβ40, NfL, and GFAP together in one reaction, from just 12.5μL of plasma, a fraction of the volume required for singleplex testing.

Download the application note to see how using 101 well-characterized samples from the Bio-Hermes-001 study, our multiplexed panel achieved an AUC of 0.919 for predicting amyloid PET status, all on a standard 96-well qPCR instrument

FAQ

Which biomarkers are most important for Alzheimer's disease today?

pTau-217, Aβ42, Aβ40, NfL, and GFAP are among the most widely studied blood-based biomarkers for Alzheimer’s disease. pTau-217 and the Aβ42/Aβ40 ratio are closely tied to amyloid and tau pathology, while NfL reflects neuronal injury and GFAP reflects astrocytic activation, together offering a broader view of disease-related changes than any single marker alone.

Neurodegenerative disease involves multiple, overlapping biological processes. Measuring several biomarkers from the same sample, rather than one at a time, can offer a fuller picture of disease stage and progression, since different markers often become abnormal at different points in the disease course.

Blood-based biomarker testing has advanced significantly and can now provide clinically meaningful insight into amyloid and tau pathology. It’s increasingly used alongside, and in some contexts as an accessible alternative to, PET imaging and CSF analysis, particularly for research, screening, and monitoring applications where routine access to those methods is limited.

See the full menu of SPLASH protein biomarkers for Alzheimer’s and CNS diseases

From singleplex to five-plex, explore every configuration available for Alzheimer’s Disease biomarker quantification.